When making a knockout cell line using CRISPR, western blotting is a useful tool to help in the verification of the cell line. However, correct interpretation of results is crucial. Here we summarize the possible fates of a protein after knockout and explore how best to validate a knockout cell line
4 result(s)
Detecting proteins with confidence starts with antibody validation. Here, we've outlined the key points.
Review of publications using HAP1 cells to set up validation assays beyond the typical western blot.
CRISPR technology now allows genes and molecular pathways to be examined with greater definition. We look at how knockout cell lines, either together with gene rescue and replication of disease mutations or as an independent cell model, can be used to validate your research and extend your findings